全部商品分类
干细胞培养基
细胞培养耗材
信号通路调控
分离鉴定试剂
细胞治疗产品
细胞健康检测
样本冷冻储藏
内胚层干细胞
中胚层干细胞
外胚层干细胞
iPS干细胞
干细胞免疫
分子生物学
表观遗传学
干细胞实验室
干细胞技术
微生物培养基

您的浏览历史

400-021-02388
您现在的位置: 首页 > 干细胞资讯 > SO资讯 > 科研进展 > 干细胞临床前研究 > 人源肝脏细胞及iPS向肝脏细胞定向分化的解决方案提供者
人源肝脏细胞及iPS向肝脏细胞定向分化的解决方案提供者
干细胞搜网 / 2017-03-28

 

人源肝脏细胞及iPS向肝脏细胞定向分化的解决方案提供者
 
iPS向肝脏细胞定向分化操作系统
(Cellartis iPS Cell to Hepatocyte Differentiation System)
以肝脏细胞为模型的实验越来越受到重视,在肝脏病变机理研究、药物代谢研究、药物毒理评估等方面有广阔的应用前景。然而,以个体原代肝细胞为材料来源会产生如取材困难、材料批间差大、无法长期稳定供应等一系列难以克服的问题。因此,通过iPS批量转化生产肝细胞的方法就显示出特别的优势。Cellartis iPS Cell to Hepatocyte Differentiation System可以稳定地、规模化地实现iPS向hepatocyte的诱导转化生产,而且诱导转化出的肝细胞能够稳定表达药物代谢相关酶系统和药物转运系统。
Cellartis iPS Cell to Hepatocyte Differentiation System是一套Do-It-Yourself系统,客户既可以对某个特殊病人来源的iPS实现转化,也可以利用cellartis供应的iPS进行标准化操作。使用本产品的过程起始于诱导人iPS转化成为确定性内胚层(Definitive Endoderm (DE))细胞,然后诱导转化DE至肝细胞。本产品是All-In-One型产品,实验所需的培养基、基质(coating)等包括在内。应用本产品转化约3 × 106 hiPS cells,可以获得约5 × 106 肝细胞(大约50 cm2面积的单层贴壁细胞)。
利用Cellartis iPS Cell to Hepatocyte Differentiation System转化得到的肝细胞除了清晰表现出肝细胞的必备特征外(如肝细胞特异性标志物、CYP系列酶等),还保留了原始细胞供体者的遗传基因背景。另外,相比较从组织切除获得的原代肝脏细胞(通常需要低温保存),通过本产品系统转化获得的肝细胞在更长的时间跨度上保持着生理功能的稳定性。这一点对于毒理学评估和病毒感染评估具有显著的意义。为长时间维持培养hiPS转化而来的肝细胞,我们推荐Cellartis Hepatocyte Maintenance Medium (Cat. No. Y30051)。
  
Cellartis iPS Cell to Hepatocyte Differentiation System 产品特点
· Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS
   cell lines, so no need to optimize for your lines.
· Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS
   cell-derived hepatocytes with diverse genetic backgrounds.
· Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver
   disease models.
· Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.
· Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.
 
■ 产品成分
· Cellartis DEF-CS 100 Culture System (Y30020, not sold separately)
· Cellartis Definitive Endoderm Differentiation Kit (Y30030, not sold separately)
· Cellartis Hepatocyte Differentiation Kit (Y30050)
 
Code No.ProductSize
Y30055Cellartis® iPS Cell to Hepatocyte Differentiation System1 Kit
Y30051Cellartis® Hepatocyte Maintenance Medium100 ml
 
 
Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).
 
Cellartis iPS Cell to Hepatocyte Differentiation System 产品订购清单
Y30050Cellartis® Hepatocyte Differentiation Kit
Y30051Cellartis® Hepatocyte Maintenance Medium
Y30035Cellartis® Definitive Endoderm Differentiation Kit with DEF-CS™
组份-1Cellartis DEF-CS 100 Culture System (Cat. No. Y30020
组份-2Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030
Y30055Cellartis DEF-CS 100 Culture System (Cat. No. Y30020,
Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030
Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050
"Cat. No. Y30020 Package Contents
Package 1:
· 100 ml DEF-CS 100 Basal Medium
· 800 μl DEF-CS COAT-1
Package 2:
· 300 μl DEF-CS GF-1
· 100 μl DEF-CS GF-2
· 40 μl DEF-CS GF-3
 
Cat. No. Y30030 Package Contents
· 10 ml Definitive Endoderm Differentiation Coating
· 18 ml Definitive Endoderm Differentiation Day 0
· 18 ml Definitive Endoderm Differentiation Day 1
· 18 ml Definitive Endoderm Differentiation Day 2
· 25 ml Definitive Endoderm Differentiation Day 3
· 47 ml Definitive Endoderm Differentiation Day 4
· 47 ml Definitive Endoderm Differentiation Day 6
 
Cat. No. Y30050 Package Contents
· 1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml)
· 1 bottle Hepatocyte Progenitor Medium (2) (50 ml)
· 2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml)
· 1 bottle Hepatocyte Maturation Medium Supplement (3B) (2.6 ml)
· 3 bottles Hepatocyte Maintenance Medium (4) (50 ml)
· 1 tube Hepatocyte Coating (7.5 ml)"
 
 
参考文献:
Asplund, Annika, et al.One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells. Stem Cell Rev and Re
 
 

评论

共(0)条评论用户评论

  • 暂时还没有任何用户评论
总计 0 个记录,共 1 页。 第一页 上一页 下一页 最末页
扫描二维码