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Hospira 6%羟乙基淀粉HES Hetastarch 羟乙基淀粉氯化钠 6% Hetastarch in 0.9% Sodium Chloride BA740193英文名称:

Hospira 6%羟乙基淀粉HES Hetastarch 羟乙基淀粉氯化钠 6% Hetastarch in 0.9% Sodium Chloride BA740193
  • Hospira 6%羟乙基淀粉HES Hetastarch 羟乙基淀粉氯化钠 6% Hetastarch in 0.9% Sodium Chloride BA740193
英文名称:
产品应用: 干细胞分离,治疗低血容量的指示,白细胞分离等
中国俗称: 羟乙基淀粉 中国简称: HES
注册品牌: Hospira 生产厂家: 美国Hospira厂家
订购货号: 0409-7248-03 保存温度: 常温
规格型号: 500mL/袋
产品种类: 干细胞产品能
到货时间: 1~5天
产品编码: YS-SO-0001372
市场价格: ¥0元
会员价格: 登录后查看
金牌价格: 登录后查看
购买数量: 单价单位

产品描述

产品中文名称:6%羟乙基淀粉
产品英文名称:6% Hetastarch in 0.9% Sodium Chloride

 

Hospira6%羟乙基淀粉简介:
6% HETASTARCH
in 0.9% Sodium Chloride Injection
For intravenous use only

Hospira 6%羟乙基淀粉HES禁忌CONTRAINDICATIONS:

• Do not use hydroxyethyl starch (HES) products, including HESPAN®, in critically ill adult patients, including patients with sepsis, due to increased risk of mortality and renal replacement therapy. (RRT) (4)
• Do not use HES products, including HESPAN®, in patients with severe liver disease (4)
• Do not use HES products, including HESPAN®, in patients with known hypersensitivity to hydroxyethyl starch (4)
• Do not use HES products, including HESPAN®, in clinical conditions where volume overload is a potential problem. (4)
• Do not use HES products, including HESPAN®, in patients with pre-existing coagulation or bleeding disorders (4)

Hospira 羟乙基淀粉HES Hetastarch 羟乙基淀粉氯化钠 警告和注意事项WARNINGS AND PRECAUTIONS:
• Avoid use in patients with pre-existing renal dysfunction (5.1)
• Discontinue use of HESPAN® at the first sign of renal injury (5.1)
• Continue to monitor renal function in hospitalized patients for at least 90 days as use of RRT has been reported up to 90 days after administration of HES products, including HESPAN® (5.1)
• HESPAN® is not recommended for use as a cardiac bypass pump prime, while the patient is on cardiopulmonary bypass, or in the immediate period after the pump has been discontinued because of the risk of increasing coagulation abnormalities and bleeding in patients whose coagulation status is already impaired. Discontinue use of HESPAN® at first sign of coagulopathy (5.2)
• Monitor liver function in patients receiving HES products, including HESPAN® (5.2)

Hospira 羟乙基淀粉HES Hetastarch 羟乙基淀粉氯化钠 6% Hetastarch in 0.9% Sodium Chloride适应症和用法INDICATIONS AND USAGE:

• HESPAN® is a hetastarch indicated for treatment of hypovolemia when plasma volume expansion is desired. (1)
• HESPAN® in leukapheresis has shown to be safe and efficacious in improving the harvesting and increasing the yield of granulocytes by centrifugal means. (1)

6%羟乙基淀粉沉淀红细胞红细胞,羟乙基淀粉,分层,血中):

1 HES自然沉降法: 即肝素抗凝血与6%HES按5:1混合,自然沉降60min,取上层液400×g离心10min,以RPMI1640洗两遍。
或者自然沉降30min,取上层液体按2:1加到淋巴细胞分离液(相对密度:1.077)的液面上层,以460g离心25分钟,采集界面层细胞,以磷酸盐缓冲液(PBS)或RPMI1640洗涤2次。

2 HES离心沉淀法: 6%的羟乙基淀粉溶液与含抗凝剂的脐血按1:5或者1:6(羟乙基淀粉终浓度为:1.2%或者1.0%)比例完全混匀后 ,经离心,
(60×g,10℃,10min),取上层液400×g离心10min,以RPMI1640洗两次备用。

Hospira 羟乙基淀粉HES Hetastarch 羟乙基淀粉氯化钠 6% 使用事例:

分离脐带血造血干细胞(UCB-HSC)的时长期采用6%羟乙基淀粉沉降法对UCB中有核细胞进行沉降分离,获得了满意的效果,单份人脐血(60-100
ml)可回收2-4X108个有核细胞,当然分层还是明显的,您所看到的所谓分层不明显我的分析可能是由于采用本法获得的上层液中还混有不少的RBC的缘故,不过可以通过其后RBC裂解液处理以清除RBC,这不影响其后分离的有核细胞的活力和生物学特性。我摸索的具体的方法为:
1. UCB按4:1的比例加入6%HES,混匀, 22oC下静置 45 min至红细胞完全沉降。分离界面出现后,吸取富含有核细胞(NCs)的上层液。
2. 将收集的富含NCs上层液按1 : 3的比例加入D-Hanks, 1500rpm 离心8 min。 弃上清,沉淀中加入RBC裂解液(不要将沉淀用任何液体悬浮后再加RBC裂解液,直接将RBC裂解液加在细胞沉淀中,15ml离心管中加5ml,50ml离心管中加20ml),4度下作用8 min。1200 rpm 离心5 min,细胞沉淀再用含 15%FCS的RPMI 1640洗涤、悬浮,350目尼龙膜过滤。镜下计数NCs细胞,台盼蓝染色观察细胞活力(细胞活力应>90%)
 

注册品牌 Hospira,订购货号 0409-7248-03,

Hospira 6%羟乙基淀粉HES Hetastarch 羟乙基淀粉氯化钠 6% Hetastarch in 0.9% Sodium Chloride BA740193

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Hospira 6%羟乙基淀粉HES Hetastarch 羟乙基淀粉氯化钠 6% Hetastarch in 0.9% Sodium Chloride BA740193

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