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您现在的位置: 首页 > 干细胞技术 > 检测试剂盒 > ELISA试剂盒 > BioGems 人APRIL预包被ELISA试剂盒,Human April Pre-Coated ELISA Kit

BioGems 人APRIL预包被ELISA试剂盒,Human April Pre-Coated ELISA Kit英文名称:

BioGems 人APRIL预包被ELISA试剂盒,Human April Pre-Coated ELISA Kit
  • BioGems 人APRIL预包被ELISA试剂盒,Human April Pre-Coated ELISA Kit
英文名称:
产品应用: 检测APRIL
中国俗称: BioGems 人APRIL预包被ELISA试剂盒 中国简称: 人APRIL ELISA试剂盒
注册品牌: BioGems 生产厂家: BioGems
订购货号: BGK2QBA2 保存温度:
规格型号: 1 Kit
产品种类: ELISA试剂盒
到货时间:
产品编码:
市场价格: ¥5740元
会员价格: 登录后查看
金牌价格: 登录后查看
购买数量: 单价单位

产品描述

 APRILTNF超家族的一员,在单核细胞,巨噬细胞,某些转化的细胞系,某些结肠癌和淋巴组织中表达。 APRIL与另一个TNF家族成员BAFF竞争两种受体,TACIBCMAAPRIL具有刺激各种肿瘤细胞系(包括Jurkat T细胞和MCF-7癌细胞)增殖的能力。像BAFF一样,APRIL也刺激BT细胞的增殖。人类APRIL基因编码至少四种选择性剪接的transcriptional variants,其产生APRIL前体蛋白的不同亚型。所有型都可以被furin蛋白酶剪切以释放可溶性C末端片段,其包含APRIL前体的TNF样受体结合位点

BioGems人类APRIL预包被ELISA(酶联免疫吸附测定)试剂盒是采用固相免疫测定法,专门设计用于检测APRIL,其中96微板预包被APRIL特异小鼠单克隆抗体。它含有NS0表达的重组APRIL。检测抗体是APRIL特异生物素化山羊多克隆抗体。该试剂盒是即用型试剂盒,已经过分析验证。

 

Human April Pre-Coated ELISA Kit分析

 

 

产品参数Product Details

Assay Type:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity:

<10pg/ml

Range:

156pg/ml-10,000pg/ml

Specificity:

Natural and recombinant Human APRIL

Uniprot:

Q2QBA2

Interference:

No detectable cross-reactivity with other relevant proteins

Storage Conditions:

Store the unopened product at 2 - 8 C.

 

组份Components

Description

Quantity

Volume

Anti-Human April Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human April Standard

2

10 ng vial

Human April Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

 

可重复性Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

653

2945

5633

864

3214

5366

Standard Deviation

26.8

156.1

219.7

42.4

202.5

268.3

Consistency (%CV)

4.1

5.3

3.9

4.9

6.3

5

 

Catalog Number : BGK2QBA2

Description

APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of the colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, competes for two receptors, TACI and BCMA. APRIL has the ability to stimulate proliferation of various tumor cell lines, including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF-like receptor binding of the APRIL precursor.

The BioGems Human APRIL Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure APRIL with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for APRIL . It contains NS0-expressed recombinant APRIL . The detection antibody is a biotinylated goat polyclonal antibody specific for APRIL . The kit is analytically validated with ready to use reagents.

 

注册品牌 BioGems,订购货号 BGK2QBA2,

BioGems 人APRIL预包被ELISA试剂盒,Human April Pre-Coated ELISA Kit

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BioGems 人APRIL预包被ELISA试剂盒,Human April Pre-Coated ELISA Kit

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