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您现在的位置: 首页 > 干细胞技术 > 检测试剂盒 > ELISA试剂盒 > BioGems 人FAS预包被ELISA试剂盒,Human FAS Pre-Coated ELISA Kit

BioGems 人FAS预包被ELISA试剂盒,Human FAS Pre-Coated ELISA Kit英文名称:

BioGems 人FAS预包被ELISA试剂盒,Human FAS Pre-Coated ELISA Kit
  • BioGems 人FAS预包被ELISA试剂盒,Human FAS Pre-Coated ELISA Kit
英文名称:
产品应用: 人FAS ELISA检测
中国俗称: 人FAS预包被ELISA试剂盒 中国简称: 人FAS ELISA
注册品牌: BioGems 生产厂家: BioGems
订购货号: BGK25445 保存温度:
规格型号: 1 Kit
产品种类:
到货时间:
产品编码:
市场价格: ¥5180元
会员价格: 登录后查看
金牌价格: 登录后查看
购买数量: 单价单位

产品描述

 

 FasFas配体(FasL)属于TNF超家族,分别是I型和II型跨膜蛋白。FasLFas的结合触发携带Fas细胞的凋亡。凋亡机制涉及通过称为FADDadaptor分子募集pro-caspase 8蛋白酶,随后将酶原处理成活性形式。然后这些活性caspases蛋白酶切割各种细胞底物,导致最终的细胞死亡。sFasR能够通过充当FasL诱发的decoy受体来抑制FasL诱导的细胞凋亡(sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL)。

 

BioGems人类FAS预包被ELISA(酶联免疫吸附测定)试剂盒是一种固相免疫测定法,专门设计用于测量FAS,其带有FAS特异预包被鼠单克隆抗体的96孔微孔板。它含有NS0表达的重组FAS。检测抗体是FAS特异的生物素化山羊多克隆抗体。该试剂盒是即用型试剂盒,已经过分析验证。

 

Human FAS Pre-Coated ELISA试剂盒分析

 

Product Details

 

 

Assay Type分析类型:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity灵敏度:

<3pg/ml

Range范围:

31.2pg/ml-2000pg/ml

Specificity特异性:

Natural and recombinant Human FAS

Uniprot:

P25445

Interference干扰:

No detectable cross-reactivity with other relevant proteins

Storage Conditions保存:

Store the unopened product at 2 - 8

 

Components组份

 

Description

数量

Volume

Anti-Human FAS Pre-coated 96-well strip microplate

抗人FAS预包被 96孔微板

1

12 strips of 8 wells

Human FASt Standard 标准样品

2

10 ng vial

Human FAS Biotinylated antibody (100x)

FAS生物素化抗体

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x) 

亲和素 - 生物素 - 过氧化物酶复合物

1

130 µL

Sample Diluent样品稀释剂

1

30mL

Antibody Diluent 抗体稀释剂

1

12mL

Avidin-Biotin-Peroxidase Diluent

亲和素 - 生物素 - 过氧化物酶稀释剂

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

洗涤缓冲液浓缩物

1

Pack

Color Developing Reagent

彩色显影剂

1

10mL

Stop Solution

终止液

1

10mL

Plate Sealers封口机

4

Each

 

Reproducibility重复性

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

138

828

1507

146

839

1512

Standard Deviation

5.8

25.67

70.83

10.95

45.31

83.16

Consistency (%CV)

4.2

3.1

4.7

7.5

5.4

5.5

 

 

Catalog Number : BGK25445

Description

Fas and Fas Ligand (FasL) belong to the TNF superfamily, and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL.

 

The BioGems Human FAS Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure FAS with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for FAS. It contains NS0-expressed recombinant FAS. The detection antibody is a biotinylated goat polyclonal antibody specific for FAS. The kit is analytically validated with ready to use reagents.

注册品牌 BioGems,订购货号 BGK25445,

BioGems 人FAS预包被ELISA试剂盒,Human FAS Pre-Coated ELISA Kit

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BioGems 人FAS预包被ELISA试剂盒,Human FAS Pre-Coated ELISA Kit

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