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您现在的位置: 首页 > 干细胞技术 > 检测试剂盒 > ELISA试剂盒 > BioGems 人上皮调节蛋白预包被试剂盒,Human Epiregulin Pre-Coated ELISA kit

BioGems 人上皮调节蛋白预包被试剂盒,Human Epiregulin Pre-Coated ELISA kit英文名称:

BioGems 人上皮调节蛋白预包被试剂盒,Human Epiregulin Pre-Coated ELISA kit
  • BioGems 人上皮调节蛋白预包被试剂盒,Human Epiregulin Pre-Coated ELISA kit
英文名称:
产品应用: 人上皮调节蛋白ELISA检测
中国俗称: 人上皮调节蛋白预包被ELISA试剂盒 中国简称: 人上皮调节蛋白ELISA
注册品牌: BioGems 生产厂家:
订购货号: BGK14944 保存温度:
规格型号: 1 Kit
产品种类:
到货时间:
产品编码:
市场价格: ¥5880元
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金牌价格: 登录后查看
购买数量: 单价单位

产品描述

 上皮调节蛋白是与EGFRErbB1)和ErbB4特异性结合但不结合ErbB2ErbB3EGF相关生长因子。 它主要表达于胎盘和外周血白细胞,以及膀胱,肺,肾和结肠的某些癌症中。上皮调节蛋白刺激角质形成细胞,肝细胞,成纤维细胞和血管平滑肌细胞的增殖。它还抑制几种肿瘤来源的上皮细胞系的生长。

 

BioGems Human Epiregulin Pre-Coated ELISA(酶联免疫吸附测定)试剂盒是采用固相免疫测定法,专门设计用于检测Epiregulin96微板预包被Epiregulin特异性小鼠单克隆抗体。 它含有大肠杆菌表达的重组Epiregulin。 检测抗体是对Epiregulin特异的生物素化山羊多克隆抗体。该试剂盒经过分析验证,是即用型试剂。

 

Product Details

Assay Type:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity:

<10pg/ml

Range:

156pg/ml-10,000pg/ml

Specificity:

Natural and recombinant Human Epiregulin

Uniprot:

O14944

Interference:

No detectable cross-reactivity with other relevant proteins

Storage Conditions:

Store the unopened product at 2 - 8 .

 

Components

Description

Quantity

Volume

Anti-Human Epiregulin Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human Epiregulin Standard

2

10 ng vial

Human Epiregulin Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

 

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

1.15

2.52

5.81

1.63

2.87

5.66

Standard Deviation

0.059

0.113

0.273

0.096

0.146

0.35

Consistency (%CV)

5.2

4.5

4.7

5.9

5.1

6.2

 

Description

Epiregulin is an EGF-related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes, as well as in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines.

The BioGems Human Epiregulin Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Epiregulin with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for Epiregulin. It contains E. coli-expressed recombinant Epiregulin. The detection antibody is a biotinylated goat polyclonal antibody specific for Epiregulin. The kit is analytically validated with ready to use reagents.

注册品牌 BioGems,订购货号 BGK14944,

BioGems 人上皮调节蛋白预包被试剂盒,Human Epiregulin Pre-Coated ELISA kit

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BioGems 人上皮调节蛋白预包被试剂盒,Human Epiregulin Pre-Coated ELISA kit

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