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您现在的位置: 首页 > 干细胞技术 > 检测试剂盒 > ELISA试剂盒 > BioGems 人神经调节蛋白预包被ELISA试剂盒,Human Neuregulin Pre-Coated ELISA kit

BioGems 人神经调节蛋白预包被ELISA试剂盒,Human Neuregulin Pre-Coated ELISA kit英文名称:

BioGems 人神经调节蛋白预包被ELISA试剂盒,Human Neuregulin Pre-Coated ELISA kit
  • BioGems 人神经调节蛋白预包被ELISA试剂盒,Human Neuregulin Pre-Coated ELISA kit
英文名称:
产品应用: 人神经调节蛋白ELISA检测
中国俗称: 人神经调节蛋白预包被ELISA试剂盒 中国简称: 人神经调节蛋白ELISA
注册品牌: BioGems 生产厂家: BioGems
订购货号: BGK02297 保存温度:
规格型号: 1 Kit
产品种类: ELISA
到货时间:
产品编码:
市场价格: ¥5810元
会员价格: 登录后查看
金牌价格: 登录后查看
购买数量: 单价单位

产品描述

 神经调节蛋白/调蛋白是由选择性剪接基因(NRG1NRG2NRG3NRG4)衍生的结构相关多肽生长因子家族。迄今为止,有超过14种来源于NRG1基因的可溶性和跨膜蛋白。跨膜NRG1同种型的胞外域的蛋白水解加工释放可溶性生长因子。 HRG1-β1含有Ig结构域和EGF样结构域;后者对于直接结合受体酪氨酸激酶erb3erb4是必需的。该结合诱导erb3erb4erb2异源二聚化,刺激导致酪氨酸磷酸化的内在激酶活性。

BioGems人类神经调节蛋白预包被ELISA(酶联免疫吸附测定)试剂盒是一种固相免疫测定,专门设计用于测量Neuregulin96孔带板,其预先包被有特异于神经调节蛋白的小鼠单克隆抗体。它含有大肠杆菌表达的重组Neuregulin。检测抗体是特异于Neuregulin的生物素化山羊多克隆抗体。该试剂盒经过分析验证,是即用型试剂。

 

 

 

Product Details

Assay Type:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity:

<10pg/ml

Range:

62.5pg/ml-4000pg/ml

Specificity:

Natural and recombinant Human Neuregulin

Uniprot:

Q02297

Interference:

No detectable cross-reactivity with other relevant proteins

Storage Conditions:

Store the unopened product at 2 - 8

 

Components

Description

Quantity

Volume

Anti-Human Neuregulin Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Human Neuregulin Standard

2

10 ng vial

Human Neuregulin Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

Reproducibility

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

675

1308

2147

694

1382

2503

Standard Deviation

28.35

61.48

113.8

40.25

84.3

172.7

Consistency (%CV)

4.2

4.7

5.3

5.8

6.1

6.9

 

Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms releases soluble growth factors. HRG1-β1 contains an Ig domain and an EGF-like domain; the latter is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity that leads to tyrosine phosphorylation.

The BioGems Human Neuregulin Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Neuregulin with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for Neuregulin. It contains E. coli-expressed recombinant Neuregulin. The detection antibody is a biotinylated goat polyclonal antibody specific for Neuregulin. The kit is analytically validated with ready to use reagents.

注册品牌 BioGems,订购货号 BGK02297,

BioGems 人神经调节蛋白预包被ELISA试剂盒,Human Neuregulin Pre-Coated ELISA kit

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BioGems 人神经调节蛋白预包被ELISA试剂盒,Human Neuregulin Pre-Coated ELISA kit

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