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您现在的位置: 首页 > 干细胞技术 > 检测试剂盒 > ELISA试剂盒 > BioGems鼠CD40配体预包被ELISA试剂盒,Murine CD40 Ligand Pre-Coated ELISA Kit

BioGems鼠CD40配体预包被ELISA试剂盒,Murine CD40 Ligand Pre-Coated ELISA Kit英文名称:

BioGems鼠CD40配体预包被ELISA试剂盒,Murine CD40 Ligand Pre-Coated ELISA Kit
  • BioGems鼠CD40配体预包被ELISA试剂盒,Murine CD40 Ligand Pre-Coated ELISA Kit
英文名称:
产品应用: 鼠CD40配体 ELISA检测
中国俗称: 鼠CD40配体预包被ELISA试剂盒 中国简称: 鼠CD40配体 ELISA
注册品牌: 生产厂家: BioGems
订购货号: BGK27548 保存温度:
规格型号: 1 Kit
产品种类: ELISA
到货时间:
产品编码:
市场价格: ¥6090元
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金牌价格: 登录后查看
购买数量: 单价单位

产品描述

 Catalog Number :BGK27548

 

CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE.

The BioGems Murine CD40 Ligand Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure CD40 Ligand with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for CD40 Ligand. It contains NS0-expressed recombinant CD40 Ligand. The detection antibody is a biotinylated goat polyclonal antibody specific for CD40 Ligand. The kit is analytically validated with ready to use reagents.

 

CD40TNF受体家族的一员,是在B细胞,树突状细胞,单核细胞,胸腺上皮细胞上表达的细胞表面蛋白,在T细胞上表达水平低。CD40信号在B细胞的增殖和分化中起重要作用,并且对于免疫球蛋白(Ig类型转换class switching)起关键作用。膜锚定的CD40配体几乎完全只表达在活化的CD4 + T淋巴细胞。未能表达CD40L会导致IgM免疫缺陷,这是一种以未能产生IgGIgAIgE为特征的疾病。

 

BioGemsCD40配体预包被ELISA(酶联免疫吸附试验)试剂盒采用固相免疫测定法,专门设计用于检测CD40配体,并在96微孔板上预包被CD40配体特异性小鼠单克隆抗体。它含有NS0表达的重组CD40配体。检测抗体是CD40配体特异的生物素化山羊多克隆抗体。该试剂盒经过分析验证,是即用型试剂。

 

 

Product Details

 

Assay Type:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity:

<10pg/ml

Range:

62.5pg/ml-4000pg/ml

Specificity:

Natural and recombinant Murine CD40 Ligand

Uniprot:

P27548

Interference:

No detectable cross-reactivity with other relevant proteins

Storage Conditions:

Store the unopened product at 2 - 8 .

 

Components 组份

Description

Quantity

Volume

Anti-Murine CD40 Ligand Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine CD40 Ligand Standard

2

10 ng vial

Murine CD40 Ligand Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

 

Reproducibility 可重复性

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

537

1562

3025

562

1675

3140

Standard Deviation

28.5

71.9

172.4

36.53

97.15

207.24

Consistency (%CV)

5.3

4.6

5.7

6.5

5.8

6.6

注册品牌 ,订购货号 BGK27548,

BioGems鼠CD40配体预包被ELISA试剂盒,Murine CD40 Ligand Pre-Coated ELISA Kit

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BioGems鼠CD40配体预包被ELISA试剂盒,Murine CD40 Ligand Pre-Coated ELISA Kit

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